Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae

Table 2 Differentially expressed proteins related to energy metabolism induced by 5 mM vanillin stress in BY4741

ORF	Protein name	Description	DEPs ratio^a
ATP biosynthetic process
YPR020W	Atp20	F1F0 ATP synthase subunit g	1.37
YKL016C	Atp7	F1F0 ATP synthase subunit d	1.33
YPL271W	Atp15	F1F0 ATP synthase subunit epsilon	1.43
YLR295C	Atp14	F1F0 ATP synthase subunit h	2.04
YPL036W	Pma2	H(+)-exporting P2-type ATPase	1.39
YDR322C-A	Tim11	F1F0 ATP synthase subunit e	1.35
Electron transport and membrane-associated energy conservation
YGR183C	Qcr9	Ubiquinol-cytochrome-c reductase subunit 9	1.43
YPR191W	Qcr2	Ubiquinol-cytochrome-reductase	1.33
YBL045C	Cor1	Ubiquinol-cytochrome-reductase	1.34
YGL187C	Cox4	Cytochrome c oxidase subunit IV	1.37
YLR038C	Cox12	Cytochrome c oxidase subunit VIb	1.32
YFR033C	Qcr6	Ubiquinol-cytochrome-c reductase subunit 6	1.31
YLR395C	Cox8	Cytochrome c oxidase subunit VIII	1.38
Glycolysis/gluconeogenesis
YGR254W	Eno1	Phosphopyruvate hydratase ENO1	1.52
YJL052W	Tdh1	Glyceraldehyde-3-phosphate dehydrogenase	1.84
YGL253W	Hxk2	Hexokinase 2	1.53
YDL021W	Gpm2	Phosphoglycerate mutase family	1.41
YJR009C	Tdh2	Glyceraldehyde-3-phosphate dehydrogenase	1.69
YGL062W	Pyc1	Pyruvate carboxylase 1	1.31
Pentose-phosphate shunt, oxidative branch
YHR183W	Gnd1	Phosphogluconate dehydrogenase	1.64
YHR163W	Sol3	6-Phosphogluconolactonase	1.51

BY4741 cultivated with 5 mM vanillin for 15 h or without vanillin for 6 h when OD₆₀₀ reached to 1.6
^aDEPs ratio (BY4741with/without vanillin stress) which is less than 1 means down-regulation; P-value ≤ 0.05

ISSN: 1475-2859