Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae

Cao, Wenyan; Zhao, Weiquan; Yang, Bolun; Wang, Xinning; Shen, Yu; Wei, Tiandi; Qin, Wensheng; Li, Zailu; Bao, Xiaoming

doi:10.1186/s12934-021-01633-z

Table 1 Differentially expressed genes/proteins related to ribosomal proteins, ribosome biogenesis and rRNA processing induced by 5 mM vanillin stress in BY4741

From: Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae

ORF	Protein/gene	Description	DEPs ratio^a/DEGs log₂ ratio^b
Ribosomal proteins at protein level			DEPs ratio
YDR025W	Rps11a	Ribosomal 40S subunit protein S11A	0.724
YPR132W	Rps23b	Ribosomal 40S subunit protein S23B	0.753
YLR388W	Rps29a	Ribosomal 40S subunit protein S29A	0.660
YDL061C	Rps29b	Ribosomal 40S subunit protein S29B	0.610
YNL096C	Rps7b	Ribosomal 40S subunit protein S7B	0.763
YKL006W	Rpl14a	Ribosomal 60S subunit protein L14A	0.695
YHL001W	Rpl14b	Ribosomal 60S subunit protein L14B	0.709
YGR148C	Rpl24b	Ribosomal 60S subunit protein L24B	0.727
YBL092W	Rpl32	Ribosomal 60S subunit protein L32	0.755
YPL143W	Rpl33a	Ribosomal 60S subunit protein L33A	0.656
YOR234C	Rpl33b	Ribosomal 60S subunit protein L33B	0.644
YIL052C	Rpl34b	Ribosomal 60S subunit protein L34B	0.707
YMR194W	Rpl36a	Ribosomal 60S subunit protein L36A	0.752
YPL249C-A	Rpl36b	Ribosomal 60S subunit protein L36B	0.743
YLR185W	Rpl37a	Ribosomal 60S subunit protein L37A	0.740
YLR325C	Rpl38	Ribosomal 60S subunit protein L38	0.719
YNL162W	Rpl42a	Ribosomal 60S subunit protein L42A	0.765
YPR043W	Rpl43a	Ribosomal 60S subunit protein L43A	0.700
YPL198W	Rpl7b	Ribosomal 60S subunit protein L7B	0.683
YHL033C	Rpl8a	Ribosomal 60S subunit protein L8A	0.746
rRNA processing at protein level
YDR365C	Esf1	Nucleolar protein involved in pre-rRNA processing	0.742
YBL018C	Pop8	Ribonuclease P	0.750
YER126C	Nsa2	rRNA-processing protein NSA2	0.727
YDR279W	Rnh202	Ribonuclease H2 subunit	0.710
YJR063W	Rpa12	RNA polymerase I core subunit RPA12	0.750
YOR341W	Rpa190	RNA polymerase I core subunit RPA190	0.746
YDR045C	Rpc11	RNA polymerase III core subunit RPC11	0.730
YLR009W	Rlp24	Ribosome biosynthesis protein	0.719
Ribosome biogenesis at mRNA level			DEGs log₂ ratio
YOL077C	Brx1	Ribosome biogenesis protein	− 1.4
YAL025C	Mak16	Ribosome biosynthesis protein	− 1.2
YOL041C	Nop12	Involved in biogenesis of large 60S ribosomal subunit	− 1.3
YPL043W	Nop4	mRNA-binding ribosome biosynthesis protein	− 1.0
YDR496C	Puf6	Negative regulation of translation, ribosomal large subunit biogenesis	− 1.5
YHR066W	Ssf1	rRNA-binding ribosome biosynthesis protein	− 1.1
YPL226W	New1	ATP binding cassette protein	− 1.1
YGR159C	Nsr1	Required for pre-rRNA processing and ribosome biogenesis	− 1.4
YDR398W	Utp5	Subunit of U3-containing small subunit processome complex	− 1.0
YDR299W	Bfr2	rRNA-processing protein	− 1.3
YNL112W	Dbp2	DEAD-box ATP-dependent RNA helicase	− 1.5
YAL025C	Mak16	Ribosome biosynthesis protein	− 1.2
YOL041C	Nop12	Nucleolar protein involved in pre-25S rRNA processing	− 1.3

BY4741 cultivated with 5 mM vanillin for 15 h or without vanillin for 6 h when OD₆₀₀ reached to 1.6
^aDEPs ratio (BY4741with/without 5 mM vanillin stress) which is less than 1 means down-regulation at protein level; P-value ≤ 0.05
^blog2 ratio (BY4741with/without 5 mM vanillin stress) which is less than 0 means down-regulation at mRNA level. False discovery rate (FDR) ≤ 0.001

Back to article page

ISSN: 1475-2859

Contact us

Submission enquiries: journalsubmissions@springernature.com

Microbial Cell Factories

Contact us