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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Proteomic analysis revealed the roles of YRR1 deletion in enhancing the vanillin resistance of Saccharomyces cerevisiae

Fig. 5

Protein levels of Tma17, Haa1 and Mbf1 in BY4741(yrr1Δ, pJFE1), BY4741(yrr1Δ, pJFE1-YRR1) and the control strain BY4741(pJFE1). Each protein had a 6×his tag at its C-terminal. Yeast cells were cultivated at 30 °C, 200 rpm in SC-URA liquid medium for 6 h when OD600 reached to 1.6. Then the cultures were collected for ELISA. Data are presented as the means ± standard errors of independent triplicate experiments. The set “No his tag” was a negative control of samples without 6×His tag for comparison. *P value < 0.001 and **P value < 0.05 in significance analysis using two-tailed Student t test

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