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Table 2 Bacterial strains and plasmids used in this study

From: Production of trans-cinnamic acid by whole-cell bioconversion from l-phenylalanine in engineered Corynebacterium glutamicum

Strains/plasmids

Description

References

E. coli XL1-Blue

recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacIqZDM15 Tn10 (Tetr)]

Stratagenea

C . glutamicum ATCC 13032

The wild-type strain

ATCC

Plasmids

  

 pHCMS

pCES-PLPV derivative; parB nonsense mutation, KmR

[39]

 pHB-104

pTak15k derivative, Ptac, SmPAL

[36]

 pHCMS-SmPAL

pHCMS derivative; PH36, RBS (in front of gene 10 of the T7 phage), SmPAL, KmR

This study

  1. aStratagene Cloning Systems, La Jolla, CA, USA