Strains/plasmids | Description | References |
---|---|---|
E. coli XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F´ proAB lacIqZDM15 Tn10 (Tetr)] | Stratagenea |
C . glutamicum ATCC 13032 | The wild-type strain | ATCC |
Plasmids | Â | Â |
 pHCMS | pCES-PLPV derivative; parB nonsense mutation, KmR | [39] |
 pHB-104 | pTak15k derivative, Ptac, SmPAL | [36] |
 pHCMS-SmPAL | pHCMS derivative; PH36, RBS (in front of gene 10 of the T7 phage), SmPAL, KmR | This study |