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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Production of trans-cinnamic acid by whole-cell bioconversion from l-phenylalanine in engineered Corynebacterium glutamicum

Fig. 1

Expression of the gene encoding for SmPAL in C. glutamicum for t-CA production. a Schematic of conversion of l-Phe to t-CA by PAL. b Schematic diagram of the plasmid pHCMS-SmPAL constructed for the expression of the gene encoding for SmPAL in C. glutamicum. PH36, strong synthetic promoter; rrnB T1T2, transcriptional terminator; rep, replication origin of C. glutamicum; p15a, replication origin of E. coli. c SDS-PAGE analysis for the expression of the gene encoding for SmPAL in C. glutamicum harboring pHCMS-SmPAL. Lane M, molecular weight markers (kDa); Lane T, total protein fractions; Lane S, soluble protein fractions; Lane I, insoluble protein fractions. Arrowhead indicates the band of SmPAL

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Microbial Cell Factories

ISSN: 1475-2859

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