Fig. 3
From: Effects of acuC on the growth development and spinosad biosynthesis of Saccharopolyspora spinosa
Expression levels of ssgA, whiA and bldD and spinosad production related to biomass in S. spinosa, S. spinosa-ΔacuC and S. spinosa-acuC. A The mRNA samples were isolated from the wild-type and mutant strains on 72 h, the expression of bldD exhibited a significant up-regulated in S. spinosa-acuC, whereas whiA and ssgA was down-regulated. The 16S rRNA gene was used as an internal control to quantify the relative expression of the target genes. Gene expression differences are shown by the bar height. Error bars indicated the standard errors of results from n = 3 replicates. * and ** indicated P < 0.05 and P < 0.01, respectively. B The process development formation rate of spinosad related to biomass in S. spinosa, S. spinosa-ΔacuC and S. spinosa-acuC