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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Functional analysis of multiple nifB genes of Paenibacillus strains in synthesis of Mo-, Fe- and V-nitrogenases

Fig. 5

Schematic map and nitrogenase activity of the ΔnifBHDK and ΔnifBHDKEN mutants of P. polymyxa and the complementary strains carrying nifB1anfHDGK, nifB2anfBHDGK, nifB2E2N2anfBHDGK of P. forsythia T98, respectively and the complementary strains carrying nifB1vnfHDGK, nifB2vnfHDGK, nifB2vnfHDGKEN of P. zanthoxyli JH29, respectively. a Schematic map of the P. polymyxa ΔnifBHDK and P. polymyxa ΔnifBHDKEN mutants and the complementary strains. b The nitrogenase activity of the P. polymyxa ΔnifBHDK and P. polymyxa ΔnifBHDKEN mutants and the complementary strains. Activity was measured by acetylene reduction assay. The complementary strains carrying nifB1anfHDGK, nifB2anfBHDGK and nifB2E2N2anfBHDGK were cultivated in Fe-dependent conditions. The complementary strains carrying nifB1vnfHDGK, nifB2vnfHDGK and nifB2vnfHDGKEN were cultivated in V-dependent conditions. Error bars indicate the SD observed from at least three independent experiments

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