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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Expression of antibody fragments in Saccharomyces cerevisiae strains evolved for enhanced protein secretion

Fig. 4

Comparison of protein secretion capacity in different mutant strains. Schematic illustration of antibody fragments (Nan (a), Pex (d) and Ran (g)). Supernatants from cultures of the antibody-expressing strains were collected after 72 h of cultivation and then processed. Western blot using anti-6x-His-tag antibody was performed under reducing (Nan (b) and Pex (e)) or nonreducing (Ran (h)) conditions. Data are expressed as average values ± SD of biological triplicates. The original images of the western blots are shown in Additional file 1: Fig. S7. For each protein, the secretion capacity in three yeast strains was evaluated (Nan (c), Pex (f) and Ran (i)). ELISA signals (absorbance values are displayed as OD450) for different recombinants were obtained with anti-6x-His-tag monoclonal antibody. The HA strain carrying empty plasmid CPOTud was used as the negative control. Results are shown as average values ± SD of biological triplicates (Nan, CPOTud) and duplicates (Pex and Ran)

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