Fig. 3
The binding activity of secreted antibody fragments in S. cerevisiae. a Schematic illustration of the sandwich ELISA format used here. b The binding activity of protein Nan to its corresponding antigen lysozyme from chicken egg white. c The binding activity of protein Pex to its corresponding antigen complement C5 from human serum. d The binding activity of protein Ran to its corresponding antigen human recombinant VEGF protein. Supernatants from HA.Nan, HA.Pex, HA.Ran and control HA.CPOTud strains were collected after 72 h of cultivation in SD-2xSCAA medium. Three antibody fragments were detected by ELISA signals (absorbance values are displayed as OD450) using anti-6x-His-tag monoclonal antibody, HA.CPOTud as a negative control. Results are average values ± SD of biological triplicates (Nan) and duplicates (Pex and Ran)