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Table 1 Chromosome copy numbers previously reported for different Synechocystis (sub)strains

From: Comparison of alternative integration sites in the chromosome and the native plasmids of the cyanobacterium Synechocystis sp. PCC 6803 in respect to expression efficiency and copy number

Synechocystis (sub)strain

OD750 or growth phase

Chromosome copy numbers per cell

Method

Reference

GTa

0.1/0.6/Stat

142/47/43

RT-qPCR

[31]

GT-Pb

0.3–0.5

9

Flow cytometry

[79]

0.1/7.0

6/2.5

Flow cytometry

[80]

GT-Wc

0.3–0.5

10

Flow cytometry

[79]

0.1/0.5/2.5

22/18/6

RT-qPCR

[6]

0.5

21

Spectroscopic

(GT-)Kazusa

1.0 (A580)

12

Colorimetric

[81]

0.1/0.5/2.5

14/7/1

RT-qPCR

[6]

0.5

10

Spectroscopic

(GT-)Micheld

0.1/0.5/2.5

19/15/8

RT-qPCR

[6]

0.5

22

Spectroscopic

PCC-Me

0.1/0.6/Stat

218/58/58

RT-qPCR

[31]

0.1/0.5/2.5

23/18/4

RT-qPCR

[6]

0.5

18

Spectroscopic

N.A.

5

Flow cytometry

[82]

(PCC-)Rippka

0.1/0.5/2.5

21/6/6

RT-qPCR

[6]

0.5

15

Spectroscopic

(PCC-)Hagemann

0.1/0.5/2.5

19/12/4

RT-qPCR

[6]

0.5

20

Spectroscopic

N.A.

Exp

5–6

Flow cytometry

[83]

N.A.

 ~ 5.0

11

RT-qPCR

[42]

  1. a GT: glucose tolerant strain
  2. b GT-P: GT strain obtained from the laboratory of Prof. Peter Nixon (Imperial College London)
  3. c GT-W: GT strain obtained from the laboratory of Prof. Wim Vermaas (Arizona State University)
  4. d (GT-)Michel: GT strain obtained from the laboratory of Prof. Klaus-Peter Michel (Bielefeld University)
  5. e PCC-M: Pasteur Culture Collection motile strain obtained from the lab of Prof. Sergey Shestakov (Moscow State University)
  6. N.A.: Not assigned, Stat: Stationary phase; Exp: Exponential phase