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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Comparison of alternative integration sites in the chromosome and the native plasmids of the cyanobacterium Synechocystis sp. PCC 6803 in respect to expression efficiency and copy number

Fig. 5

Determination of relative syfp2 copy number for the different constructs introduced in Synechocystis. The syfp2 copy numbers were determined for each strain in reference to the chromosome copy numbers (i.e. normalized against the endogenous genes petB, and rrn16S) by RT-qPCR. The slr0944 (arsB) locus was selected as the control condition representing relative expression equal to one. The box-whisker plot represents three independent experimental trials with technical triplicates for all the strains (control strains indicated with *). One-way ANOVA test was performed to evaluate significant differences (P < 0.05 denoted by ***)

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Microbial Cell Factories

ISSN: 1475-2859

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