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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Comparison of alternative integration sites in the chromosome and the native plasmids of the cyanobacterium Synechocystis sp. PCC 6803 in respect to expression efficiency and copy number

Fig. 2

Quantitative analysis of the generated Synechocystis expression strains, showing the sYFP2 fluorescence signal recorded at the timepoints 0, 2, 4, 6, 24 and 48 h after induction with 1 mM IPTG The experiment was repeated three times, using six independent replicates (red line; n = 6), three independent replicates ( blue line; n = 3), or a single culture (black line; n = 1) for each strain AJ. The values were always measured in three technical replicates, normalized to OD750, and used for calculating the plotted averages and the standard deviations. The initial rates of fluorescence increase, calculated based on three most consistent data points between 0–6 h and averaged between the three parallel runs, have been shown in grey dotted lines. The horizontal grey bar shows the 20,000–40,000 fluorescence level range to visualize the scale difference between AH and I, J. The control strains have been indicated by *. Negative WT reference is presented in Additional file 1: Fig. S4.

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