Biodegradation of flonicamid by Ensifer adhaerens CGMCC 6315 and enzymatic characterization of the nitrile hydratases involved

Table 2 Substrate specificities of purified CnhA and PnhA

Substrate	CAS Registry No	Specific activity ± Standard deviation (U/mg)
Substrate	CAS Registry No	CnhA	PnhA
FLO	158062-67-0	4.30 ± 0.12b	34.12 ± 1.32c
ACE	135,410-20-7	0.85 ± 0.01a	21.34 ± 0.17b
THI	111988-49-9	7.65 ± 0.04c	58.09 ± 1.38d
3-CP	100-54-9	158.25 ± 3.86e	625.62 ± 4.24e
IAN	771-51-7	21.28 ± 0.79d	9.15 ± 0.39a
Fipronil	120068-37-3	ND	ND
Dichlobenil	1194-65-6	ND	ND
Bromoxynil	1689-84-5	ND	ND

NHase activity was assayed with 2 mmol/L substrate. Data indicate the means of three replicates. Values are the mean ± SD. Different letters adjacent to the values indicate statistically significant differences (Duncan test, p ≤ 0.05)
ND: no activity detected

ISSN: 1475-2859