Biodegradation of flonicamid by Ensifer adhaerens CGMCC 6315 and enzymatic characterization of the nitrile hydratases involved

Table 1 Biochemical properties of purified enzymes from FLO-degrading bacteria

Name	Protein	Microorganism	V_{max (U/mg)}	K_{m (mM)}	Optimum reaction		References
Name	Protein	Microorganism	V_{max (U/mg)}	K_{m (mM)}	pH	Temperature	References
NHase	PnhA	E. adhaerens CGMCC 6315	88.7	2.96	6	50 °C	This study
NHase	CnhA	E. adhaerens CGMCC 6315	9.55	5.07	8	50 °C	This study
NHase	PnhF	M. flocculans CGMCC 1.16731	5.9	32.9	5	50 °C	Zhao et al. [16]
NHase	ANHase	Aminobacter sp. CGMCC 1.17253	14.98 × 10^–3	21.03	7	40 °C	Yang et al. [17]
Nitrilase	NitA	A. faecalis CGMCC 17553	0.58	0.59	7	40 °C	Yang et al. [15]
Nitrilase	NitD	A. faecalis CGMCC 17553	0.18 × 10^–3	145.87	8	40 °C	Yang et al. [15]

ISSN: 1475-2859