Fig. 5
From: Early response of methanogenic archaea to H2 as evaluated by metagenomics and metatranscriptomics
Comparison of metatranscriptomic and qPCR results of selected genes affected by early H2 treatment. The threshold value of significant gene expression was set to fold change 2 in gene expression (log2FC = 1). The selected genes are from bin_1: ppdK (pyruvate, phosphate dikinase); bin_6: mfnF ((4-{4-[2-(gamma-L-glutamylamino)ethyl]phenoxymethyl}furan-2-yl)methanamine synthase), cofG (7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase), rplJ (ribosomal protein L10), eno (enolase); bin_27: cdhC (acetyl-CoA decarboxylase/synthase), mcrB (methyl-CoM reductase beta subunit), frhA (Coenzyme F420 hydrogenase subunit alpha); bin_35: mcrC (methyl-CoM reductase gamma subunit); bin_59: gapA (glyceraldehyde 3-phosphate dehydrogenase), oppA (peptide nickel transport system substrate binding protein). Blue columns: metatranscriptomic expression, red columns: qPCR results