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Table 5 Fractionation of the S. pombe cell wall polysaccharides using recombinant endo-β(1,3)-d-glucanase PfLam16A

From: Analysis and application of a suite of recombinant endo-β(1,3)-d-glucanases for studying fungal cell walls

Total cell walla

Polysaccharide

% of cell wall polysaccharideb

α-glucan

Galactomannoprotein

β-glucanc

28.60 ± 1.13

14.50 ± 1.40

56.90 ± 2.34

β(1,3)-d-glucanc

β(1,6)-d-glucanc

53.63 ± 2.22

3.28 ± 3.13

  1. aThe % of total cell wall in the cell is 35.74 ± 1.01
  2. bValues are the average from three independent experiments (mean ± SD)
  3. cβ-glucan is the sum of β(1,3)-d-glucan plus β(1,6)-d-glucan
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Microbial Cell Factories

ISSN: 1475-2859

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