Skip to main content

Table 4 Comparison between the enzymatic cell wall degradations with PfLam16A and Quantazyme

From: Analysis and application of a suite of recombinant endo-β(1,3)-d-glucanases for studying fungal cell walls

Enzyme

Enzyme units or weight (µg)a

% of cell wall degradation

Standard deviation (SD)

PfLam16Ab

20 µg

53.63

± 2.22

Quantazymec

400 unitsd

53.76

± 1.29

  1. aEnzyme conditions (units or weight) provided by the commercial supplier
  2. bThe value is the average from seven independent experiments (mean ± SD). PfLam16A reaction conditions: 100 mM Sodium phosphate buffer, pH 6.5, 20 h, 70 °C. % of degradation in each of the seven experiments: 56.59%, 51.97%, 51.20%, 54.42%, 49.93%, 55.32%, 55.94%
  3. cThe value is the average from three independent experiments (mean ± SD). Quantazyme reaction conditions: 33.5 mM potassium phosphate monobasic/KOH buffer, pH 7.5, 60 mM β-mercaptoethanol, 24 h, 37 °C. % of cell wall degradation in each of the three experiments: 54.40%, 51.82%, 55.05%
  4. dOne unit of Quantazyme activity is defined as the amount of enzyme required to produce a 0.001 decrease in A800 per minute at pH 7.5 and 25 °C using a suspension of brewer’s yeast (Saccharomyces cerevisiae) as substrate