Analysis and application of a suite of recombinant endo-β(1,3)-d-glucanases for studying fungal cell walls

Table 2 Percentage of cell wall degradation with combinations of two recombinant endo-β(1,3)-d-glucanase enzymes

Recombinant enzyme 1	Recombinant enzyme 2	Enzymes 1 + 2 Units or weight (µg)	Reaction conditions			% of cell wall degradation^a
Recombinant enzyme 1	Recombinant enzyme 2	Enzymes 1 + 2 Units or weight (µg)	Buffer	pH	Temp. (°C)	% of cell wall degradation^a
E-LAMHV^b	TmLam16A^c	50 U + 50 µg	Sodium phosphate 50 mM	5.5	50	42.10 ± 0.09
E-LAMHV^b	ZgLam16A^c	50 U + 10 µg	Sodium phosphate 50 mM	6.5	40	41.69 ± 1.09
BhLam81A^c	E-LAMHV^b	10 µg + 50 U	Sodium phosphate 50 mM	5.5	50	44.51 ± 0.36
BhLam81A^c	CtLam81A^c	10 µg + 150 µg	Sodium phosphate 50 mM	6.5	60	33.87 ± 0.33
CtLam81A^c	E-LAMHV^b	150 µg + 50 U	Sodium phosphate 50 mM	5.5	50	67.77 ± 1.92
CtLam81A^c	TmLam16A^c	150 µg + 50 µg	Sodium phosphate 50 mM	6.5	60	54.80 ± 1.83
CtLam81A^c	ZgLam16A^c	150 µg + 10 µg	Sodium phosphate 50 mM	6.5	50	47.77 ± 7.28
CtLic16A^c	E-LAMHV^b	75 µg + 50 U	Sodium phosphate 50 mM	5.5	50	34.43 ± 0.37
CtLic16A^c	CtLam81A^c	75 µg + 150 µg	Sodium phosphate 50 mM	6.5	60	38.71 ± 3.63
PfLam16A^c	TmLam16A^c	40 µg + 50 µg	Sodium phosphate 100 mM	6.5	70	59.40 ± 3.43
PfLam16A^c	TmLam16A^c	40 µg + 50 µg	Sodium phosphate 50 mM	7.0	60	44.33 ± 1.04

^aPercentage of cell wall degradation after 36 h of reaction (mean ± SD). All reactions were carried out from at least two independent experiments
^bCommercial supplier: Megazyme
^cCommercial supplier: NZYTech

ISSN: 1475-2859