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Table 1 Cell wall degradation by specific recombinant endo-β(1,3)-d-glucanases

From: Analysis and application of a suite of recombinant endo-β(1,3)-d-glucanases for studying fungal cell walls

Commercial suppliers

Recombinant enzyme

Reaction conditions

% of cell wall degradation

Source organism

  

Specified by the supplier

Maximum degradation a

Buffer

pH

Temp. (°C)

Incubation time (h)

Enzyme units or weight (µg)

MP Biomedicals

Quantazyme

Potassium phosphate monobasic/KOH 33.5 mM + β-mercaptoethanol 60 mM

7.5

37

25

400 Ub

53.76 ± 1.29

Oerskovia xanthineolytica

Megazyme

E-LAMHV

Sodium acetate 100 mM

5.0

40

25

100 Uc

68.17 ± 0.15

Hordeum vulgare (barley)

E-LICACT

Sodium phosphate 100 mM

6.5

60

36

120 Ud

59.28 ± 0.20

Clostridium thermocellum

NZYTech

ALam55A

Sodium acetate 50 mM

5.0

45

25

50 µg

37.39 ± 2.04

Arthrobacter sp.

BhLam81A

Sodium phosphate 50 mM

6.5

60

36

10 µg

17.59 ± 1.32

Bacillus halodurans

CtLam81A

Sodium phosphate 50 mM

6.0

65

25

150 µg

54.37 ± 6.47

Clostridium thermocellum

CtLic16A

MES 50 mM

6.0

65

36

35 µg

19.70 ± 6.18

Clostridium thermocellum

PfLam16A

Sodium phosphate 100 mM

6.5

70

36

50 µg

53.17 ± 2.56

Pyrococcus furiosus

TmLam16A

Sodium phosphate 50 mM

7.0

45

36

50 µg

34.44 ± 0.89

Thermotoga maritima

TnLam16A

Sodium phosphate 50 mM

6.0

70

25

50 µg

66.75 ± 5.01

Thermotoga neapolitana

TpLam16A

Sodium phosphate 50 mM

6.0

80

36

50 µg

68.25 ± 1.35

Thermotoga petrophila

ZgLam16A

Glycine–NaOH 100 mM

8.5

40

36

10 µg

19.52 ± 0.36

Zobellia galactanivorans

Prokazyme

Bglu110

Sodium phosphate 100 mM

7.0

75

36

10 Ue

78.23 ± 6.15

Rhodothermus marinus

  1. aReaction condition in which each enzyme exhibits the maximum % of cell wall degradation (mean ± SD from at least two independent experiments). In addition to these conditions, each enzyme was tested according to the conditions described in Additional file 3: Table S3
  2. bOne unit of Quantazyme activity is defined as the amount of enzyme required to produce a 0.001 decrease in A800 per minute from a suspension of brewer’s yeast (Saccharomyces cerevisiae) as substrate in 33.5 mM potassium phosphate monobasic buffer, pH 7.5 with KOH, 60 mM β-mercaptoethanol at 25 °C
  3. cOne unit of E-LAMHV activity is defined as the amount of enzyme required to release one µmole of glucose-reducing sugar equivalents per minute from laminarin β(1,3)-d-glucan (10 mg/mL) as substrate in 100 mM sodium acetate buffer, pH 5.0 at 40 °C
  4. dOne unit of E-LICACT activity is defined as the amount of enzyme required to release one µmole of glucose-reducing sugar equivalents per minute from barley β-d-glucan (5 mg/mL) as substrate in 100 mM sodium phosphate buffer, pH 6.5 at 40 °C
  5. eOne unit of Bglu110 activity is defined as the amount of enzyme required to release one µmole of glucose-reducing sugar equivalents per minute from lichenan β(1,3)(1,4)-d-glucan (10 mg/mL) as substrate in 100 mM sodium phosphate buffer, pH 7.0 at 75 °C