Commercial suppliers | Recombinant enzyme | Reaction conditions | % of cell wall degradation | Source organism | ||||
---|---|---|---|---|---|---|---|---|
Specified by the supplier | Maximum degradation a | |||||||
Buffer | pH | Temp. (°C) | Incubation time (h) | Enzyme units or weight (µg) | ||||
MP Biomedicals | Quantazyme | Potassium phosphate monobasic/KOH 33.5 mM + β-mercaptoethanol 60 mM | 7.5 | 37 | 25 | 400 Ub | 53.76 ± 1.29 | Oerskovia xanthineolytica |
Megazyme | E-LAMHV | Sodium acetate 100 mM | 5.0 | 40 | 25 | 100 Uc | 68.17 ± 0.15 | Hordeum vulgare (barley) |
E-LICACT | Sodium phosphate 100 mM | 6.5 | 60 | 36 | 120 Ud | 59.28 ± 0.20 | Clostridium thermocellum | |
NZYTech | ALam55A | Sodium acetate 50 mM | 5.0 | 45 | 25 | 50 µg | 37.39 ± 2.04 | Arthrobacter sp. |
BhLam81A | Sodium phosphate 50 mM | 6.5 | 60 | 36 | 10 µg | 17.59 ± 1.32 | Bacillus halodurans | |
CtLam81A | Sodium phosphate 50 mM | 6.0 | 65 | 25 | 150 µg | 54.37 ± 6.47 | Clostridium thermocellum | |
CtLic16A | MES 50 mM | 6.0 | 65 | 36 | 35 µg | 19.70 ± 6.18 | Clostridium thermocellum | |
PfLam16A | Sodium phosphate 100 mM | 6.5 | 70 | 36 | 50 µg | 53.17 ± 2.56 | Pyrococcus furiosus | |
TmLam16A | Sodium phosphate 50 mM | 7.0 | 45 | 36 | 50 µg | 34.44 ± 0.89 | Thermotoga maritima | |
TnLam16A | Sodium phosphate 50 mM | 6.0 | 70 | 25 | 50 µg | 66.75 ± 5.01 | Thermotoga neapolitana | |
TpLam16A | Sodium phosphate 50 mM | 6.0 | 80 | 36 | 50 µg | 68.25 ± 1.35 | Thermotoga petrophila | |
ZgLam16A | Glycine–NaOH 100 mM | 8.5 | 40 | 36 | 10 µg | 19.52 ± 0.36 | Zobellia galactanivorans | |
Prokazyme | Bglu110 | Sodium phosphate 100 mM | 7.0 | 75 | 36 | 10 Ue | 78.23 ± 6.15 | Rhodothermus marinus |