Table 1 Bacterial strains and plasmids used in this study
Name | Characteristics | Sources |
|---|---|---|
Strains | ||
 E. coli BL21(DE3) | F− ompT hsdSB(rB− mB−) gal dcm(DE3) | Invitrogen |
 E. coli BW25113 | lacIqrrnBT14ΔlacZWJ16 hsdR514 ΔaraBADAH33 ΔrhaBADLD78 | NBRP-E. coli at NIG |
 BWH18 | BW25113, ΔfeaB::Ptrc-aroGfbr-pheAfbr, ΔtyrA | [51] |
 BTR01 | BL21(DE3) with pQFA01 and pQFA02 | This study |
 BTR02 | BL21(DE3) with pQFA01 and pQFA03 | This study |
 BTR03 | BL21(DE3) with pQFA01 and pQFA04 | This study |
 BTR04 | BL21(DE3) with pQFA01 and pQFA05 | This study |
 BTR05 | BL21(DE3) with pQFA01 and pQFA06 | This study |
 BTR06 | BL21(DE3) with pQFA07 and pQFA02 | This study |
 BTR07 | BL21(DE3) with pQFA08 and pQFA02 | This study |
 BTR08 | BTR01 with pQFA09 | This study |
 BTR09 | BTR01 with pQFA10 | This study |
 BTR10 | BTR01 with pQFA11 | This study |
 BTR11 | BTR01 with pQFA12 | This study |
 BTR12 | BTR01 with pQFA13 | This study |
 BTR13 | BWH18 with T7 RNA polymerase gene in the chromosome | This study |
 BTR14 | BTR13, ∆ptsG | This study |
 BTR15 | BTR14, ∆pykA | This study |
 BTR16 | BTR15, ∆pykF | This study |
 BTR17 | BTR14, ∆tyrR | This study |
 BTR18 | BTR15, ∆tyrR | This study |
 BTR19 | BTR16, ∆tyrR | This study |
 BTR20 | BTR13 with pQFA01, pQFA02 and pQFA10 | This study |
 BTR21 | BTR14 with pQFA01, pQFA02 and pQFA10 | This study |
 BTR22 | BTR15 with pQFA01, pQFA02 and pQFA10 | This study |
 BTR23 | BTR16 with pQFA01, pQFA02 and pQFA10 | This study |
 BTR24 | BTR17 with pQFA01, pQFA02 and pQFA10 | This study |
 BTR25 | BTR18 with pQFA01, pQFA02 and pQFA10 | This study |
 BTR26 | BTR19 with pQFA01, pQFA02 and pQFA10 | This study |
 BTR27 | BTR16 with PT7-EcPPTase fragment integrated into the locus between entD and insL1 | This study |
 BEL09 | BTR27, pCDF-CaER, pQFA02, pQFA10 | This study |
 BTR28 | BTR27 with pQFA10 and pQFA14 | This study |
 BTR29 | BTR27 with pQFA15 and pQFA02 | This study |
 BTR30 | BTR27 with pQFA15 and pQFA16 | This study |
 BTR31 | BTR27 with pQFA17 and pQFA18 | This study |
 BTR32 | BTR27 with pQFA19 and pQFA20 | This study |
 BTR33 | BTR27 with pQFA21 and pQFA22 | This study |
 BTR34 | BTR27 with pQFA21 and pQFA16 | This study |
Plasmids | ||
 pCDFDuet-1 | CDF ori with PT7; StrR | Novagen |
 pETDuet-1 | ColE1 ori with PT7; AmpR | Novagen |
 pRSFDuet-1 | RSF ori with PT7; KanR | Novagen |
 pREDCas9 | pSC101ts, pLac-λ-Red, Cas9, ParaBAD -gRNA-bla SpcR | [62] |
 pGRB | pUC18 for gRNA construction | [62] |
 pQFA01 | pCDFDuet-1 harboring genes encoding CaER and EcPPTase | This study |
 pQFA02 | pETDuet-1 harboring the gene encoding SruCAR | This study |
 pQFA03 | pETDuet-1 harboring the gene encoding MsCAR | This study |
 pQFA04 | pETDuet-1 harboring the gene encoding SroCAR | This study |
 pQFA05 | pETDuet-1 harboring the gene encoding TtCAR | This study |
 pQFA06 | pETDuet-1 harboring the gene encoding NcCAR | This study |
 pQFA07 | pCDFDuet-1 harboring genes encoding CaER and BsPPTase | This study |
 pQFA08 | pCDFDuet-1 harboring genes encoding CaER and NiPPTase | This study |
 pQFA09 | pRSFDuet-1 harboring the gene encoding TpPAL1 | This study |
 pQFA10 | pRSFDuet-1 harboring the gene encoding AtPAL2 | This study |
 pQFA11 | pRSFDuet-1 harboring the gene encoding ZmPAL2 | This study |
 pQFA12 | pRSFDuet-1 harboring the gene encoding RgTAL | This study |
 pQFA13 | pRSFDuet-1 harboring the gene encoding SriPAL | This study |
 pQFA14 | pETDuet-1 harboring genes encoding SruCAR and CaER | This study |
 pQFA15 | pRSFDuet-1 harboring genes encoding AtPAL2 and CaER | This study |
 pQFA16 | pCDFDuet-1 harboring the gene encoding SruCAR | This study |
 pQFA17 | pETDuet-1 harboring the gene encoding AtPAL2 | This study |
 pQFA18 | pRSFDuet-1 harboring genes encoding SruCAR and CaER | This study |
 pQFA19 | pETDuet-1 harboring genes encoding SruCAR and AtPAL2 | This study |
 pQFA20 | pRSFDuet-1 harboring the gene encoding CaER | This study |
 pQFA21 | pETDuet-1 harboring genes encoding AtPAL2 and CaER | This study |
 pQFA22 | pRSFDuet-1 harboring the gene encoding SruCAR | This study |