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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Metabolic engineering of Escherichia coli for de novo production of 3-phenylpropanol via retrobiosynthesis approach

Fig. 5

Balancing metabolic strength of the downstream 3-phenylpropanol pathway. Various expression patterns were designed by expressing the AtPAL2, CaER and SruCAR genes at both the high (RSF ori) and middle (ColE1 ori) or low (CDF ori) copy number. The fermentation was conducted in the presence of 10 g/L glucose and 0.01 mM IPTG, and the broth was analyzed after 48 h of cultivation

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