Fig. 5From: Metabolic engineering of Escherichia coli for de novo production of 3-phenylpropanol via retrobiosynthesis approachBalancing metabolic strength of the downstream 3-phenylpropanol pathway. Various expression patterns were designed by expressing the AtPAL2, CaER and SruCAR genes at both the high (RSF ori) and middle (ColE1 ori) or low (CDF ori) copy number. The fermentation was conducted in the presence of 10Â g/L glucose and 0.01Â mM IPTG, and the broth was analyzed after 48Â h of cultivationBack to article page