Table 5 Strains and plasmids

Corynebacterium glutamicum ATCC 13032

Lab stock

E. coli DH5α

Host of plasmid

Lab stock

K. pneumoniae CGMCC 1.6366

TUAC01 Wild type, Amp^r

[7]

ΔtpiA

ΔtpiA, Apr^r

This work

ΔtpiA-ΔmgsA

ΔmgsA, ΔtpiA, Apr^r, Str^r

This work

ΔtpiA-ΔmgsA-ΔgldA

ΔmgsA, ΔtpiA, ΔgldA, Apr^r

This work

ΔtpiA-ΔmgsA-ΔgldA-ΔdhaD

ΔmgsA, ΔtpiA, ΔgldA, ΔdhaD, Apr^r, Str^r

This work

ΔtpiA-ΔdhaD

ΔdhaD,ΔtpiA, Apr^r

This work

ΔtpiA-ΔbudA

ΔbudA, ΔtpiA, Apr^r, Str^r

This work

ΔgapA

ΔgapA, Apr^r

This work

ΔgapC

ΔgapC, Apr^r

This work

ΔgapA-ΔgapC

ΔgapA, ΔgapC, Apr^r, Str^r

This work

ΔtpiA-ΔdhaK

Δdhak, ΔtpiA, Apr^r

This work

ΔtpiA-ΔdhaK1

Δdhak1, ΔtpiA, Apr^r

This work

ΔtpiA-ΔdhaK2

Δdhak2, ΔtpiA, Apr^r

This work

ΔtpiA-ΔdhaK3

Δdhak3, ΔtpiA, Apr^r

This work

ΔtpiA-ΔDHAK

Δdhak, Δdhak123, ΔtpiA, Apr^r

This work

Plasmids

 pIJ773

Apr^r, aac(3)IV with FRT sites, 4334 bp

[27]

 pIJ778

Str^r, aadA with FRT sites, 4337 bp

[27]

 pDK6

Kan^r, lacI^Q, tac, 5118 bp

[28]

 pDK6-hdpA

Kan^r, carries hdpA, 5.9kp

This work

 pDK6-red

Kan^r, carries λ-Red genes (gam, bet, exo) 7.1 kp

[29]

 pDK6-flp

Kan^r, carries the yeast FLP recombinase gene 6.3 kp

[29]

 pMD18-T-simple

Amp^r, TA cloning vector, 2692 bp

Takara

 pMD18-T-ΔtpiA

Amp^r, Apr^r, carries part of tpiA

This work

 pMD18-T-ΔmgsA

Amp^r, Str^r, carries part of mgsA

This work

 pMD18-T-ΔgldA

Amp^r, Apr^r, carries part of gldA

This work

 pMD18-T-ΔdhaD

Amp^r, Str^r, carries part of dhaD

This work

 pMD18-T-ΔgapA

Amp^r, Apr^r, carries part of gapA

This work

 pMD18-T-ΔgapC

Amp^r, Apr^r, carries part of gapC

This work