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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Genetic tools for the development of recombinant lactic acid bacteria

Fig. 3

Repetitive integration system of Lb. casei BL23. a Prophage recombinase LCABL_13040-50-60 mediated homologous recombination, resulting in the replacement of geneX with the cat marker, while site-specific recombinase Cre subsequently eliminated the marker and a loxP site was left. The inserted loxP site was verified by sequencing analysis. b The gfp gene was repetitively integrated into the chromosome between the loxP sites of the integration construct pUC-lox-cat-gfp and chromosome. 40-50-60, recombinase LCABL_13040-50–60; HU and HD, up and down homologous arms; cat, bla, indicate chloramphenicol and ampicillin resistance genes respectively

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