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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Genetic tools for the development of recombinant lactic acid bacteria

Fig. 1

Schematic representation of dsDNA recombineering in Lactobacillus casei. a A piece of dsDNA substrate harboring the lox66-cat-lox71 cassette (lox66 and lox71 sites, red; cat/Cm marker, green), gfp (the gene of the green fluorescence protein, purple), and DNA overhangs homologous to the genomic insertion site (H1, blue; H2, yellow) was electroporated into Lb. casei expressing an λ Red-like recombinase operon LCABL_13040-50-60. b Once the dsDNA substrate was integrated into the genome, the recombinant cell was endowed with chloramphenicol resistance (Cmr). The recombinant cell was transformed with a plasmid pMSPCre carrying the site specific recombinase Cre to direct the recombination between the lox66 and lox71 sites for the deletion of the Cm marker. The resultant mutant cells (Cms) contained the gfp gene and a lox72 site at the target site

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