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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Development of IL-15/IL-15Rα sushi domain-IgG4 Fc complexes in Pichia pastoris with potent activities and prolonged half-lives

Fig. 2

Pilot-scale fermentation, purification and characterization of IL-15/SuIL-15Rα-dFc and IL-15/SuIL-15Rα-mFc. a Representative three-step fermentation process, including batch phase, glycerol fed-batch phase and methanol induction phase. Parameters, such as dissolved oxygen (DO), feeding speed, temperature and pH, were monitored during fermentation. b Cell growth of the two IL-15/SuIL-15Rα-IgG4 Fc complex-expressing strains during fermentation was monitored and represented as wet cell weight. c The expression of the two IL-15/SuIL-15Rα-IgG4 Fc complexes during fermentation was analyzed by Western blotting using an anti-human IgG4-HRP conjugate under nonreducing conditions. d The downstream processing workflow of fermentation broth. e The two purified IL-15/SuIL-15Rα-IgG4 Fc complexes were separated by SDS-PAGE under reducing conditions and identified by Western blotting or Coomassie blue staining. The contents within the dashed boxes are the presumed protein structure of the corresponding bands. M: prestained protein marker; dFc: IL-15/SuIL-15Rα-dFc; mFc: IL-15/SuIL-15Rα-mFc

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