Fig. 6
From: Refactoring of a synthetic raspberry ketone pathway with EcoFlex
Level 3 assembly of the final optimised strain with a malonyl-CoA regeneration module, optimised reductase step and a pathway stability monitor. The plasmid strains were assessed for growth and eGFP fluorescence in 96-well microtitre plates, as well as LC–MS for pathway intermediates (representative of six biological repeats). Error bars represent standard deviation. The plasmid strains (pRK-B6 and pRK-B12) correspond to the B6 and B12 HBA modules, shown in Fig. 4 and Additional file 1: Table S5. The control plasmid (pTU1-A) is pTU1-D-kasOp*-sfGFP. Further details are described in methods