Fig. 7
From: The cssR gene of Corynebacterium glutamicum plays a negative regulatory role in stress responses
Negative regulation of reduce systems by CssR. a β-galactosidase analyses of the different ROS-detoxifying enzymes and the reducing systems promoter activities by using the transcriptional lacZY chromosomal fusion reporter expressed in the WT and ΔcssR mutant under normal conditions. b Quantitative RT-PCR analyses of the different ROS-detoxifying enzymes and the reducing systems expression in WT and ΔcssR mutant under indicated conditions. Results were the average of three independent experiments; the standard deviation was indicated by bars. The mRNA levels were presented relative to the value obtained from WT cells. Relative transcript levels of WT strains were set at a value of 1.0. Data shown were the averages of three independent experiments, and error bars indicated the SDs from three independent experiments. *P < 0.05. c–f CssR did not bind directly to the promoters of the genes involved in redox homeostasis. The interaction between His6-CssR and the trx promoter fragment (Ptrx) (c), the mrx1 promoter fragment (Pmrx1) (d), the mtr promoter fragment (Pmtr) (e), or the mshC promoter fragment (PmshC) (f)