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Table 3 Hydrogen peroxide stress tolerance for strains with and without β-caryophyllene production after 200 mM H2O2 exposure for 30 min

From: Adaptive laboratory evolution of β-caryophyllene producing Saccharomyces cerevisiae

Mutation

No production

With production

Strain

Relative H2O2 tolerance

Strain

Relative H2O2 tolerance

β-caryophyllene (mg/g DCW)

Control

YAG114

 

YAG118

 

3.39

CDC39

YAG119

ND

YAG132

ND

3.55

DAN4 T200T

YAG120

ND

YAG133

ND

3.47

FLO9 T288T

YAG121

+

YAG134

+

3.15

tK(CUU)C/MAK32 int

YAG122

ND

YAG135

ND

3.44

MDS3

YAG123

+

YAG136

+

2.94

MST27/tR(UCU)G1 int

YAG124

+

YAG137

+

10.27

MTL1 S257S

YAG125

+

YAG138

+

2.24

RIF1 D709N

YAG126

ND

YAG139

ND

3.36

RNR3/FIS1 int

YAG127

ND

YAG140

ND

3.31

SNO2/SNZ2 int

YAG128

ND

YAG141

ND

3.57

STE6 T1025N

YAG129

ND

YAG142

+

12.66

YAL064W-B/TDA8 int

YAG130

ND

YAG143

ND

3.35

YHL050C int

YAG131

ND

YAG144

ND

3.25

  1. Production was measured in 20 mL test tube with 3 mL culture overlayed with 500 μL dodecane. ND: not different from wild-type. +: ~ 10× higher survival compared to wild-type. Bold: p value < 0.05 using two-tailed Student’s t test compared with control