Adaptive laboratory evolution of β-caryophyllene producing Saccharomyces cerevisiae

Table 1 Mutations chosen for detailed characterization

Chromosome	Position	Mutation	Amino acid change	Gene	Abbreviation
3	286312	C→T	Nonsense mutation	CDC39	CDC39
10	715141	A→G	T200T	DAN4	DAN4 T200T
1	27105	A→G	T288T	FLO9	FLO9 T288T
3	151555	+ A	Intergenic	tK(CUU)C/MAK32	tK(CUU)C/MAK32 int
7	128474	T→A	Nonsense mutation	MDS3	MDS3
7	404475	+ G	Intergenic	MST27/tR(UCU)G1	MST27/tR(UCU)G1 int
7	530034	A→C	S257S	MTL1	MTL1 S257S
2	754982	C→T	D709N	RIF1	RIF1 D709N
9	241053	(A)_21→22	Intergenic	RNR3/FIS1	RNR3/FIS1 int
14	12986	(T)_11→14	Intergenic	SNO2/SNZ2	SNO2/SNZ2 int
11	43222	G→T	T1025N	STE6	STE6 T1025N
1	12690	A→T	Intergenic	YAL064W-B/TDA8	YAL064W-B/TDA8 int
8	2303	(C)_11→12	Intergenic	YHL050C/YHL050C	YHL050C int

ISSN: 1475-2859