Fig. 3

Isolated evolved mutants exhibited increased β-caryophyllene production over the parental strain. A Initial screening of isolated mutants from each evolved population based on relative product yield in 48 well plates. Pop8 = P2 (day 8), Pop11 = P2 (day 11), Pop14 = P2 (day 14), and Pop18 = P2 (day 18). Production was measured in 48 well plate with 1 mL culture overlayed with 166 μL dodecane. B Confirmation of product formation in the five best performing mutants. Production was measured in 20 mL test tube with 3 mL culture overlayed with 500 μL dodecane. The mutants are named first by the population they were isolated from followed by the isolate number. For example, P11M1 is the first mutant from Pop11. Asterisks: p value < 0.05 using two-tailed Student’s t-test against control