Table 1 Strains and plasmids used in this study
Description | Reference or sourcea | |
|---|---|---|
Strains | ||
E. coli JM109 | recA1 endA1 gyrA96 thi-1 hsdRl7 (rk− mk+) e14− (mcrA−) supE44 relA1 △(lac-proAB)/F’ [traD36 proAB+ laclq lacZ △M15] | TaKaRa |
C. tropicalis ATCC 20,336 | URA3/URA3 XYL2/XYL2 | ATCC |
Y. lipolytica CICC 31,251 | CICC | |
C. tropicalis XZX | ura3/ura3 XYL2/XYL2 | [19] |
C. tropicalis XZX-B1 | ura3/ura3 xyl2::gda-URA3/XYL2 | This study |
C. tropicalis XZX-B2 | ura3/ura3 xyl2::gda/XYL2 | This study |
C. tropicalis XZX-B3 | ura3/ura3 xyl2::gda/xyl2::gda-URA3 | This study |
C. tropicalis XZX-B4 | ura3/ura3 xyl2::gda/xyl2::gda | This study |
C. tropicalis XZX-B4G | ura3/ura3 xyl2::gda-URA3-PGAPDH-GND-TGAPDH/ xyl2::gda | This study |
C. tropicalis XZX-B4Z | ura3/ura3 xyl2::gda-URA3-PGAPDH-ZWF-TGAPDH/ xyl2::gda | This study |
C. tropicalis XZX-B4Zt | ura3/ura3 xyl2::gda-PGAPDH-ZWF-TGAPDH/xyl2::gda | This study |
C. tropicalis XZX-B4ZG | ura3/ura3 xyl2::gda-PGAPDH-ZWF-TGAPDH/xyl2::gda-URA3-PGAPDH-GND-TGAPDH | This study |
C. tropicalis XZX-B4M | ura3/ura3 xyl2::gda-URA3-PGAPDH-MAE-TGAPDH /xyl2::gda | This study |
Plasmids | ||
Tm-gda-URA3 | Contain a functional marker gene flanked by gda | [19] |
Ts-XYL2I-gda-URA3 | Contain the cassette for the disruption of the first XYL2 allele | This study |
Ts-XYL2II-gda-URA3 | Contain the cassette for the disruption of the second XYL2 allele | This study |
Ts-XYL2I-gda-URA3-PGAPDH-YlZWF-TGAPDH. | Contain the YlZWF gene expression cassette | This study |
Ts-XYL2II-gda-URA3-P GAPDH -YlGND-T GAPDH | Contain the YlGND gene expression cassette | This study |
Ts-XYL2I-gda-URA3-P GAPDH -YlMAE-T GAPDH | Contain the YlMAE gene expression cassette | This study |