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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Genome-based selection and application of food-grade microbes for chickpea milk fermentation towards increased l-lysine content, elimination of indigestible sugars, and improved flavour

Fig. 1

Microbial pathways for the synthesis and degradation of l-lysine. Potential l-lysine overproducers were identified from the NCC database based on the presence and absence of key genes related to the well-established routes of l-lysine metabolism [95]. Enzymes shown in yellow were essential for l-lysine biosynthesis, whereas enzymes shown in blue were involved in competing pathways and l-lysine degradation [96,97,98]. The presence of diaminopimelate decarboxylase (lysA) was regarded as essential for potential l-lysine-producing strains (level 1). Diaminopimelate epimerase (dapF) and diaminopimelate dehydrogenase (ddh) were then used to infer the putative pathway involved (level 2). DP dehydrogenase pathway, SP succinylase pathway, AP acetylase pathway, DapA 4-hydroxy-tetrahydrodipicolinate synthase, DapB 4-hydroxytetrahydrodipicolinate reductase, DapD tetrahydrodipicolinate succinylase, DapC succinyl-amino-ketopimelate transaminase, DapE N-succinyl-diaminopimelate desuccinylase, DapF diaminopimelate epimerase, Ddh diaminopimelate dehydrogenase, THDP-NAT tetrahydrodipicolinate acetylase, PatA N-acetyl-amino-ketopimelate aminotransferase, NAD-DAC N-acetyl-diaminopimelate deacetylase, LysA diaminopimelate decarboxylase, Hom homoserine dehydrogenase, MurF UDP-N-acetylmuramoylalanyl-d-glutamyl-2,6-diamino-pimelate-d-alanyl-d-alanyl ligase, EC 1.4.3.14 l-lysine oxidase, EC 5.1.1.5 lysine racemase, KamA l-lysine 2,3-aminomutase, DavB lysine 2-monooxygenase; CadA, lysine decarboxylase; LucD, lysine N6-hydroxylase

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Microbial Cell Factories

ISSN: 1475-2859

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