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Fig. 7 | Microbial Cell Factories

Fig. 7

From: In-situ muconic acid extraction reveals sugar consumption bottleneck in a xylose-utilizing Saccharomyces cerevisiae strain

Fig. 7

PCA and muconic acid production by the TN22 strain in the absence or presence of the PPG solvent (1:5 ratio). YP medium containing 10% glucose (YPD), 10% xylose (YPX) or 6% glucose and 4.5% xylose (YPDX) with 2% ethanol, buffered with 50 mM citrate buffer at initial pH of 5.5 and inoculum OD600 16. Media contained either A no PPG or B presence of PPG (solvent/fermentation medium 1:5 ratio). C Muconic acid titer and yield (aqueous phase in grey plus solvent phase in white) (at 192 h) in the absence (A) and presence (B) of PPG. Muconic acid was extracted from the PPG solvent phase using 50% ethanol. Results are means of three independent replicates. Error bars show standard deviation at each time point

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