Table 1 Primers used for quantitative real-time polymerase chain reaction
From: Bacillus subtilis revives conventional antibiotics against Staphylococcus aureus osteomyelitis
Genes | Forward primers | Reverse primers |
|---|---|---|
cna | 5′-AAAGCGTTGCCTAGTGGAGA-3′ | 5′-AGTGCCTTCCCAAACCTTTT-3′ |
clfA | 5′-ATTGGCGTGGCTTCAGTGCT-3′ | 5′-CGTTTCTTCCGTAGTTGCATTTG-3′ |
IcaA | 5′-ACACTTGCTGGCGCAGTCAA-3′ | 5′-TCTGGAACCAACATCCAACA-3′ |
sarA | 5′-TCTTGTTAATGCACAACAACGTAA-3′ | 5′-TGTTTGCTTCAGTGATTCGTTT-3′ |
argA | 5′-GAAGACGATCCAAAACAAAGAG-3′ | 5′-GTCATTCATATTTTTAGCTTGCTC-3′ |
argB | 5′-CCAGTTTGCCACGTATCTTC-3′ | 5′-GCACCATGTGCATGTCTTC-3′ |
RNAIII | 5′-GAAGGAGTGATTTCAATGG-3′ | 5′-TAAGAAAAATACATAGCACTGA-3′ |
hla | 5′-GAAAGGTACCATTGCTGGTCA-3′ | 5′-AAGGCCAGGCTAAACCACTT-3′ |
mecA | 5′-CCTCTGCTCAACAAGTTCCA-3’ | 5′-ACGTTGTAACCACCCCAAGA-3′ |
gyrB | 5′-TTATGGTGCTGGACAGATACA-3′ | 5′-CACCGTGAAGACCGCCAGATA-3′ |