From: Development of an automated platform for the optimal production of glycoconjugate vaccines expressed in Escherichia coli
Components
Periplasmic Buffer 1
Periplasmic Buffer 2
Tris.HCl
30 mM (pH 8.5)
100 mM (pH 7.4)
Sucrose
20% (w/v)
-
EDTA
1 mM
10 mM
Lysozyme
1 mg/mL (fresh)
Protease inhibitor cocktail
1 tablet/10 mL (fresh)