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Table 2 Composition of Periplasmic Buffers (1) and (2)

From: Development of an automated platform for the optimal production of glycoconjugate vaccines expressed in Escherichia coli

Components

Periplasmic Buffer 1

Periplasmic Buffer 2

Tris.HCl

30 mM (pH 8.5)

100 mM (pH 7.4)

Sucrose

20% (w/v)

-

EDTA

1 mM

10 mM

Lysozyme

1 mg/mL (fresh)

-

Protease inhibitor cocktail

1 tablet/10 mL (fresh)

-