Table 4 Oligonucleotides used as primers in this study
From: Adaptive laboratory evolution accelerated glutarate production by Corynebacterium glutamicum
Primer | Sequence (5′–3′) |
|---|---|
JJ49 | GGCAAGCTTATCCGTCCCAGTGGGCCT |
JJ50 | CCCATCCACTAAACTTAAACAGAGTCCTTGTGGTTTCAT |
JJ51 | TGTTTAAGTTTAGTGGATGGGGACCCAGCAATCAAGATCATGGAGGCAGTGAGCTAA |
JJ52 | CGGTCTAGATGCACCCAGCCTTCGCGG |
JJ108 | CATTTGGAACCGGCATGTCCC |
JJ109 | GGTGCCGGTTGCGAGGAGGATC |
GltBA | GCATGCCTGCAGGTCGACTCTAGAGCACCGTTGGACTCTATCCG |
GltBB | TCGATGGTTTGAAATGCCATG |
GltBC | CATGGCATTTCAAACCATCGA |
GltBD | AATTCGAGCTCGGTACCCGGGGATCCTGAACTCAAACAGTCCACG |
GltBE | CAACTACATGGCGCACTCTC |
GltBF | GTTCCACATCAAATCGGCGG |
gltB-for | CCTGCAGGTCGACTCTAGAGGATTCCGAAAGGAGGCCCTTCAGATGAAACCACAAGGAC |
gltB-rev | TTGATGAATCCTTGTGGGTCGGCCATTAGCTCACTGCCTC |
gltD-for | CAAGATCATGGAGGCAGTGAGCTAATGGCCGACCCACAAG |
gltD-rev | AGTGAATTCGAGCTCGGTACCCGGGCTAGACAGCCAGCGG |