Table 3 Plasmids used in this study

pEC-XT99A

Tet^R, C. glutamicum/E. coli shuttle vector (P_trc, lacI^q, pGA1 oriV_Cg)

[74]

pEC-XT99A-gabTD

pEC-XT99A expressing gabT and gabD from P. stutzeri ATCC 17588

[16]

pEC-XT99A-gabTD^P134L

pEC-XT99A expressing gabT and gabD with SNP P134L from P. stutzeri ATCC 17588

[73]

pEC-XT99A-tetA(Z)^Δ21bp-gabTD^P134L

pEC-XT99A with 21 bp off-frame deletion in tetA(Z) expressing gabT and gabD with SNP P134L from P. stutzeri ATCC 17588

This study

pEKEx3

Spec^R, C. glutamicum/E. coli shuttle vector (P_tac lacI^q pBL1, oriV_Ec)

[75]

pEKEx3-patDA

pEKEx3, expressing patD and patA from E. coli MG1655

[16]

pEKEx3-gltBD

pEKEx3, expressing gltBD from C. glutamicum ATCC 13032

This study

pEKEx3-gltB^E686QD

pEKEx3, expressing gltBD with an amino acid exchange gltB^E686Q from C. glutamicum ATCC 13032

This study

pVWEx1

Kan^R, C. glutamicum/E. coli shuttle vector (P_tac, lacI^q)

[76]

pVWEx1-ldcC

pVWEx1 expressing ldcC from E. coli MG1655

[77]

pK19mobsacB

Kan^R, mobilizable E. coli vector mutagenesis (oriV, sacB)

[78]

pK19mobsacB-gltB^E686Q

pK19mobsacB to introduce SNP gltB^E686Q

This study

pK19mobsacB-ΔgltB

pK19mobsacB for deletion of gltB from C. glutamicum ATCC 13032

This study