Skip to main content

Table 2 Bacterial strains used in this study

From: Adaptive laboratory evolution accelerated glutarate production by Corynebacterium glutamicum

Strain

Relevant characteristics

References

E. coli DH5α

∆lacU169 (φ80lacZ ∆M15), supE44, hsdR17, recA1, endA1, gyrA96, thi-1, relA1

[69]

E. coli S17-1

recA, pro, hsdR, RP4- 2Tc∷Mu Km∷Tn7 integrated into the chromosome

[71]

C. glutamicum GRLys1 (DM1933ΔCGP123)

C. glutamicum ATCC 13032 with modifications: Δpck, pycP458S, homV59A, 2 copies of lysCT311I, 2 copies of asd, 2 copies of dapA, 2 copies of dapB, 2 copies of ddh, 2 copies of lysA, 2 copies of lysE, in-frame deletion of prophages CGP1 (cg1507-cg1524), CGP2 (cg1746-cg1752) and CGP3 (cg1890-cg2071)

[72]

GRLys1ΔgltB

GRLys1 with in-frame deletion: gltB (cg0229)

This study

GSLA2

GRLys1 with in-frame deletions: sugR (cg2115), ldhA (cg3219), snaA (cg1722), cgmA (cg2893)

[16]

GSLA2G

GSLA2 with in-frame deletion: gdh (cg2280)

[16]

GSLA2::gltBE686Q

GSLA2 carrying the mutation gltBE686Q

This study

GSLA2G::gltBE686Q

GSLA2G carrying the mutation gltBE686Q

This study

GluA

GSLA2G (pVWEx1-ldcC) (pEKEx3-patDA) (pEC-XT99A-gabTD)

[16]

GluA T0

GSLA2G (pVWEx1-ldcC) (pEKEx3-patDA) (pEC-XT99A-gabTDP134L)

[73]

GluA T5

Strain evolved from GluA T0 after five transfers

This study

GluA T7

Strain evolved from GluA T0 after seven transfers

This study

GluA RG1

GSLA2G (pVWEx1-ldcC) (pEKEx3-patDA) (pEC-XT99A-tetA(Z)Δ21bp-gabTDP134L)

This study

GluA RG2

GSLA2G::gltBE686Q (pVWEx1-ldcC) (pEKEx3-patDA) (pEC-XT99A-gabTDP134L)

This study

GluA RG3

GSLA2G::gltBE686Q (pVWEx1-ldcC) (pEKEx3-patDA) (pEC-XT99A-tetA(Z)Δ21bp-gabTDP134L)

This study