From: Adaptive laboratory evolution accelerated glutarate production by Corynebacterium glutamicum
Strain | Relevant characteristics | References |
---|---|---|
E. coli DH5α | ∆lacU169 (φ80lacZ ∆M15), supE44, hsdR17, recA1, endA1, gyrA96, thi-1, relA1 | [69] |
E. coli S17-1 | recA, pro, hsdR, RP4- 2Tc∷Mu Km∷Tn7 integrated into the chromosome | [71] |
C. glutamicum GRLys1 (DM1933ΔCGP123) | C. glutamicum ATCC 13032 with modifications: Δpck, pycP458S, homV59A, 2 copies of lysCT311I, 2 copies of asd, 2 copies of dapA, 2 copies of dapB, 2 copies of ddh, 2 copies of lysA, 2 copies of lysE, in-frame deletion of prophages CGP1 (cg1507-cg1524), CGP2 (cg1746-cg1752) and CGP3 (cg1890-cg2071) | [72] |
GRLys1ΔgltB | GRLys1 with in-frame deletion: gltB (cg0229) | This study |
GSLA2 | GRLys1 with in-frame deletions: sugR (cg2115), ldhA (cg3219), snaA (cg1722), cgmA (cg2893) | [16] |
GSLA2G | GSLA2 with in-frame deletion: gdh (cg2280) | [16] |
GSLA2::gltBE686Q | GSLA2 carrying the mutation gltBE686Q | This study |
GSLA2G::gltBE686Q | GSLA2G carrying the mutation gltBE686Q | This study |
GluA | GSLA2G (pVWEx1-ldcC) (pEKEx3-patDA) (pEC-XT99A-gabTD) | [16] |
GluA T0 | GSLA2G (pVWEx1-ldcC) (pEKEx3-patDA) (pEC-XT99A-gabTDP134L) | [73] |
GluA T5 | Strain evolved from GluA T0 after five transfers | This study |
GluA T7 | Strain evolved from GluA T0 after seven transfers | This study |
GluA RG1 | GSLA2G (pVWEx1-ldcC) (pEKEx3-patDA) (pEC-XT99A-tetA(Z)Δ21bp-gabTDP134L) | This study |
GluA RG2 | GSLA2G::gltBE686Q (pVWEx1-ldcC) (pEKEx3-patDA) (pEC-XT99A-gabTDP134L) | This study |
GluA RG3 | GSLA2G::gltBE686Q (pVWEx1-ldcC) (pEKEx3-patDA) (pEC-XT99A-tetA(Z)Δ21bp-gabTDP134L) | This study |