Fig. 7

Glutarate production by C. glutamicum GluA T7 in fed-batch mode. GluA T7 was cultivated in CGXII minimal medium in fed-batch mode over 64 h, containing 40 g L⁻¹ glucose and 150 g L⁻¹ glucose from the feeding solution. Glutarate production is indicated in green squares (g L⁻¹), biomass concentration (CDW) is shown in black diamonds (g L⁻¹), glucose concentration (g L⁻¹) is plotted as pink hollow triangles, and 5AVA concentration (g L⁻¹) in red diamonds, feed solution (mL) is plotted as pink line and the relative dissolved oxygen saturation (rDOS) is indicated in light blue (%). Cultivation was performed at 30 °C and pH 7.0 regulated with 10% (v/v) H₃PO₄ and 4 M KOH. An overpressure of 0.4 bar was applied. 0.6 mL L⁻¹ of antifoam agent AF204 (Sigma Aldrich, Taufkirchen, Germany) was added to the medium manually before inoculation