Fig. 2
From: Adaptive laboratory evolution accelerated glutarate production by Corynebacterium glutamicum
Influence of the amino acid exchange GabDP134L on a growth and glutarate production and b the combined in vitro enzyme activities of GABA transaminase GabT and succinate semialdehyde dehydrogenase GabD. a Strain GluA and GluA T0 were cultivated in the BioLector microcultivation system with 40 g L−1 glucose in CGXII minimal medium supplemented with 1 mM IPTG. Supernatant concentrations of glutarate were determined after 96 h and are given as means and standard deviations of three independent cultivations. b Crude extracts of DH5α (pEC-XT99A-gabTD) and DH5α (pEC-XT99A-gabTDP134L) were assayed for combined in vitro enzyme activities of GABA transaminase GabT and succinate semialdehyde dehydrogenase GabD with 20 mM 5AVA as substrate and increasing glutarate concentrations. Statistical significance was assessed in Student’s unpaired t-test (*p < 0.05)