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Table 2 Primers used in this study

From: Genetic engineering of the precursor supply pathway for the overproduction of the nC14-surfactin isoform with promising MEOR applications

Primer

Sequence

PC-F

ATTTTTAAAGTATGTATACAAATGA

PC-R

TTATAAAAGCCAGTCATTAGGCCTA

pps-LF-F

TTTATTTGAAAGGGAAAGGCGATCC

pps-LF-R

AATGGCCTCTGTCCGCTAATCCGCTCGGATTCCCTCCAGTTCTCATAATA

pps-DR-F

TATTATGAGAACTGGAGGGAATCCGAGCGGATTAGCGGACAGAGGCCT

pps-DR-R

TCATTTGTATACATACTTTAAAAATAATGGCCTCTGTCCGCTAATCCGCT

pps-RF-F

TAGGCCTAATGACTGGCTTTTATAATTGAGCGAACATACTTATTCTTTAA

pps-RF-R

CAAGGTGCGCAGCCAGCCGGCTGGC

pks-LF-F

AGCGTATGTGATGCCAAGTATGGAG

pks-LF-R

AGAATCGCTTTTCACACTAGTGCCTAGCTTTATTGTAACAAGAAAAAAT

pks-DR-F

ATTTTTTTCTTGTTACAATAAAGCTAGGCACTAGTGTGAAAAGCGATTCT

pks-DR-R

TCATTTGTATACATACTTTAAAAATAAAATACTCTCAGAAAACAAATAC

pks-RF-F

TAGGCCTAATGACTGGCTTTTATAAATGCCAAAACAAATTGACCATGAA

pks-RF-R

TAATGAGAGTGTGTCAATGCGACTG

Pveg-bte-LF-F

TTTATGGCGGACAAAAAGGAACTGA

Pveg-bte-LF-F

AGTGTGATGCTGTGTAAGATAGATCGATTGACGCTCCTTTATACTCTGTA

Pveg-F

GATCTATCTTACACAGCATCACACT

Pveg-R

GTTTGTCCTCCTTATTAGTTAATCT

bte-F

AGATTAACTAATAAGGAGGACAAACATGGCTACAACATCTCTTGCTTCG

bte-R

ACAACAATATGGCCCGTTTGTTGAATTAAACACGAGGTTCAGCAGGGAA

Pveg-bte-DR-F

TATCCCTGCTGAACCTCGTGTTTAAATCGCATGAAAGCACATTCTCTTGA

Pveg-bte-DR-R

TCATTTGTATACATACTTTAAAAATTGAGAAAACAGCGGTATGCTGAAG

Pveg-bte-RF-F

AAATAACAGATTAAAAAAATTATAAATGTCCAAAATTATTGCAATTAAG

Pveg-bte-RF-R

TGTTTTCACCGATACCGGCAGTAAA

ackA-LF-F

TTTATGGCGGACAAAAAGGAACTGA

ackA-LF-R

CAAGAGAATGTGCTTTCATGCGATGTTTGTCCTCCTTATTAGTTAATCT

ackA-DR-F

AGATTAACTAATAAGGAGGACAAACATCGCATGAAAGCACATTCTCTTG

ackA-DR-R

TCATTTGTATACATACTTTAAAAATTGAGAAAACAGCGGTATGCTGAAT

ackA-RF-F

AAATAACAGATTAAAAAAATTATAAATGTCCAAAATTATTGCAATTAAC

ackA-RF-R

TGTTTTCACCGATACCGGCAGTAAA

amyE-up-F

AACCCGACATCCGGCGTTCTCATGG

amyE-up-R

ACAACAATATGGCCCGTTTGTTGAATCTTGACACTCCTTATTTGATTTTT

Te–F

AAAAATCAAATAAGGAGTGTCAAGATTCAACAAACGGGCCATATTGTT

Te-R

ATGTCAAAAGGAGAAGAACTTTTTA

gfp-F

ATGTCAAAAGGAGAAGAACTTTTTA

gfp-R

CGGTAAGTCCCGTCTAGCCTTGCCCTTATTTATAAAGTTCGTCCATACCG

amyE-down-R

AAGGGCAAGGCTAGACGGGACTTACCG

amyE-down-F

CACCGATGTACACGTCATCTGCAC

Pveg -F

TAAACATTCTCAAAGGGATTTCTAAGATCTATCTTACACAGCATCACAT

Pveg -R

TAAAAAGTTCTTCTCCTTTTGACAT TACATTTATTGTACAACACGAGCCC

P43-F

TAAACATTCTCAAAGGGATTTCTAAGATAGGTGGTATGTTTTCGCTTGAC

P43-R

TAAAAAGTTCTTCTCCTTTTGACATGTGTACATTCCTCTCTTACCTATAAT

  1. Underlined letters represent complementary sequences for overlap-extension PCR