Genetic engineering of the precursor supply pathway for the overproduction of the nC14-surfactin isoform with promising MEOR applications

Table 1 Bacterial strains and plasmids used in this study

Strain or plasmid	Characteristics	Source
Plasmids
pTPC	pMD19-T harboring the PC cassette	[22]
pBTE	pUC18 derivative containing cloned BTE	[21]
Strains
BSFX022	B. subtilis 168 derivative, sfp⁺yhfl⁺P₄₃-bte⁺, ΔfadE	[21]
BSFX023	BSFX022 derivative, Δpps	This work
BSFX024	BSFX023 derivative, Δpks	This work
BSFX025	BSFX024 derivative, insertion of bte under the control of the P_veg promoter, ΔackA	This work
BSFX026	BSFX024 derivative, ΔackA	This work
168 (P_veg-GFP)	B. subtilis 168 derivative, insertion of P_veg-gfp cassette at the locus of amyE, ΔamyE	This work
168 (P₄₃-GFP)	B. subtilis 168 derivative, insertion of P₄₃-gfp cassette at the locus of amyE, ΔamyE	This work

ISSN: 1475-2859