Skip to main content
Fig. 1 | Microbial Cell Factories

Fig. 1

From: Immune response induced by oral administration with a Saccharomyces cerevisiae-based SARS-CoV-2 vaccine in mice

Fig. 1

Schematic diagram and expression analysis of EBY100/pYD1-RBD. a Schematic model of EBY100/pYD1-RBD. The RBD protein was fused with Aga2, which was expressed by pYD1 as the subunit of the a-agglutinin receptor via the Gly-Ser linker. Further, Aga2 was then bound to Aga1, which is the subunit of yeast, through two disulfide bonds. b Western blot analysis. c Immunofluorescence assay. EBY100/pYD1 (left side); EBY100/pYD1-RBD (right side). (Magnification: ×400). d Flow cytometric analysis. EBY100/pYD1 (left side); EBY100/pYD1-RBD (right side, 45.1%). 10,000 cells were counted. The results from Western blot, immunofluorescence microscopy assay and flow cytometric analysis are representative of three independent experiments. e mAb-mediated binding to EBY100/pYD1-RBD detected by ELISA. The data are presented as the means ± SDs. f mAb-mediated inhibition of EBY100/pYD1-RBD binding to hACE2 analyzed by ELISA

Back to article page