Fig. 5

Engineered NRRV antigens produced in yeast are comparable to original antigens produced in bacteria. a Mean binding affinity of antigen variants measured by biolayer interferometry. Variants labeled with a star were further evaluated in animals. Error bars represent the maximum and minimum binding affinity of three technical replicates. b–d Titer of neutralizing antibodies raised against b P[8], c P[4], or d P[6], measured by fluorescence-based virus neutralization assay. Each data point represents the serum of one animal, as measured 35 days after the first injection, or 7 days after the final injection. Antigens were mixed with alhydrogel before injection. Treatment groups within each serotype were compared by ANOVA, followed by post hoc comparison of means using Tukey’s HSD test according to best practices [35]. ANOVA results were not significant for any serotype (p > 0.05), but adjusted p-values from Tukey’s test are noted