Fig. 2
From: Surface display as a functional screening platform for detecting enzymes active on PET
Functional surface display of IsPETase variants comparing a C-IgAP and LppOmpA anchor module. a Whole-cell and in-gel fluorescence for IsPETase variants (wt: wild type; A: Austin, S: Son) surface-displayed using either C-IgAP or LppOmpA module with and without L-rhamnose induction. The nanobody:GFP binding workflow is indicated. Unbound GFP was run on the outer left lane. Samples have been normalized to ODU. b Absorbance change at 405 nm measured on pNP-acetate for IsPETase variants expressed via the two modules. Active PETase degrades pNP-acetate to pNP, and acetic acid as illustrated. NC: Negative control BL21(DE3) cells not harboring the expression vector. Statistical evaluation: Multiple comparison analysis (two-way ANOVA); ns: no significant statistical difference, ****: p-value < 0.0001