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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Surface display as a functional screening platform for detecting enzymes active on PET

Fig. 1

Schematic illustration of surface display modules for IsPETase expression. C-IgAP (purple) and LppOmpA (orange) anchor module and orientation of IsPETases within the modules are shown. TEV cleavage sites and His-tags (6x histidine) are indicated. N-terminal signal sequences (lppss and pelB) precede both constructs. A schematic illustrating both modules in the bacterial outer membrane is displayed (right side), indicating the location of nanobody:GFP (gray:green) binding. The pelB signal sequence is cleaved during translocation and therefore not displayed in the schematic

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