Phosphonates enantiomers receiving with fungal enzymatic systems

Table 3 Results of the biotransformation of 1-amino-1-(3-thienyl)methylphosphonic acid (1) with Rhodotorula mucilaginosa

Biocatalyst	Cultivation medium	Time of cultivation [h]	Time of preincubation under starvation conditions [h]	Time of biotransformation [h]	e.e. ^a,b [%]
R. mucilaginosa	PDB	72	0	72	35
		72	24	72	28
		120	0	24	94
		120	24	24	96
		120	48	24	78
	PDB2	120	0	24	39
		120	48	24	≥ 98
		120	48	48	≥ 99
	YM	72	0	72	33

Bold values indicate best result
^aRacemic mixtures were enriched in (S)-enantiomer. ^bThe yield of bioconversion is adequate to the evaluated e.e. and in case of pure enantiomer synthesis the yield is 100%

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