Fig. 6

Schematic illustrating the ameliorative effect of trehalose on heat stress in P. ostreatus. Trehalose decreased the content of lactate and downregulated the expression of the key glycolytic enzymes, except hk, thus alleviating the increase in glycolysis in P. ostreatus under heat stress. In addition, trehalose increased the gene expression and enzymatic activity of G6PDH, enhanced PPP activity, and increased NADPH production. Thus, the conversion of GSSG into GSH was increased by the conversion between NADPH and NADP⁺, and greater amounts of ROS would be scavenged in cells. Moreover, trehalose alleviated the decrease in the OCR caused by heat stress. Tre: trehalose, G: glucose, HK: hexokinase, G-6-P: glucose 6-phosphate, F-1,6-2P: fructose-1,6−2 phosphate, Pyr: pyruvate, LA: lactate, EMP: glycolysis, TPS1: trehalose-6-phosphate synthase 1, G6PDH: glucose-6-phosphate dehydrogenase, PPP: pentose phosphate pathway, OCR: oxygen consumption rate. The black dashed line indicates results from other reports, the blue dashed lines indicate the omitted intermediate steps, and the blue solid lines indicate results obtained in this study