Fig. 3

Trehalose can enhance PPP activity in P. ostreatus under heat stress. The WT strains were transferred to new CYM medium with or without trehalose or sorbitol after 5 days of culture and treated with heat (40 °C). Mycelia were then collected for determination of a the relative gene expression level of g6pdh, b the enzymatic activity of G6PDH, and c the NADP⁺/NADPH ratio. The WT strain, CK strain, and tps1 overexpression strains were treated with heat (40 °C) after culture at 28 °C for 5 days and then collected for determination of d the relative gene expression level of g6pdh and e the NADP⁺/NADPH ratio. n = 3; the data are expressed as mean ± SEM values. The significance analysis was conducted within CYM, Sorbitol, or Trehalose group; within WT, CK, OE::TPS1-5, or OE::TPS1-9 group separately, and the significant differences were marked with different lowercase letters over the columns (P < 0.05 according to the Tukey or Kruskal-Wallis test), the lowercase letters among different groups have no comparability in significance analysis