Fig. 2

Trehalose can alleviate the increase in glycolysis in P. ostreatus under heat stress. The WT strains were transferred to new CYM medium with or without trehalose or sorbitol after 5 d of culture and treated with heat (40 °C). Mycelia were then collected for a the lactate content assay and measurement of the relative gene expression levels of b hk, c pfk, and d pk. The WT strain, CK strain, and tps1 overexpression strains OE::TPS1-5 and OE::TPS1-9 were treated with heat (40 °C) after culture at 28 °C for 5 d. Mycelia were then collected for determination of e the lactate content and relative gene expression levels of f hk, g pfk, and h pk. n = 3; the data are expressed as mean ± SEM values. FW: fresh weight. The significance analysis was conducted within CYM, Sorbitol, or Trehalose group; within WT, CK, OE::TPS1-5, or OE::TPS1-9 group separately, and the significant differences were marked with different lowercase letters over the columns (P < 0.05 according to the Tukey or Kruskal-Wallis test), the lowercase letters among different groups have no comparability in significance analysis